Application of HKM Vibrio parahemolyticus Chromogenic Medium

Abstract:

Vibrio parahemolyticus is a saltophilic and important food-borne pathogen which widely exists in all kinds of seafood. The general method costs more time and has low specificity in isolating and identificating of V. parahemolyticus. In order to enhance the efficiency and shorten the detection periods, a new chromogenic medium (HKMVPM) was designed in this study. The sensitivity, specificity and detection efficiency of HKMVPM were studied and compared with that of KHVPM and TCBS by detecting the reference strains and natural samples. The results showed that the selectivity and specificity of HKMVPM were same as that of KHVPM. The detection efficiency of HKMVPM was better than that of KHVPM. The detection efficiency of KHVPM was better than that of TCBS. V. parahemolyticus chromogenic medium shows a high degree of higher sensitivity and specificity and has a good prospect in applying.

Key words:Chromogenic media, Vibrio parahemolyticus, Natural samples, Application study