Screening, Identification, and Capability Assessment of a Phosphorus Solubilizing Fungus in Rhizosphere of Burma Reed

Abstract:

To reveal mechanism of soil and water conservation plant such as Burma Reed (Neyraudia reynaudiana) tolerant of low phosphorus, explore germplasm of solubilizing phosphorus and improve use efficiency of phosphorus in latosolic red soil, a solubilizing phosphate fungus FP1 was isolated from rhizosphere soil of Burma reed and identified as Aspergillus niger according to morphological characteristics and ITS rDNA sequences. The dynamic variation of solubilizing phosphate capability and pH of in the liquid medium with three kinds of inorganic phosphate indicated that the pH of the liquid medium decreased significantly after inoculated FP1 and the solubilizing phosphate capability was related with culturing time. Except for Ca3(PO4)2, solubilizing phosphate capability of FP1 rose first, then fell, and became stable in the end. The maximum phosphate solubilizing efficiencies were ordered as follows: AlPO4 (92.02%) > Ca3(PO4)2 (41.62%) > mixture of these three insoluble phosphates (35.86%) > FePO4·4H2O (19.20%). The fungus FP1 was high efficient on solubilizing AlPO4 and FePO4·4H2O. It is indicated that microorganisms with high efficient solubilizing phosphorus exists in soil and water conservation plant rhizosphere.