Cloning and Sequence Analysis of Cellulase Genes from Trichoderma longibrachiatum

Abstract:

Strain FU05 with cellulose-degrading activity was isolated from cellulose-rich environment and identified as Trichoderma longibrachiatum through morphology characteristics and ITS sequence analysis. The cellulase genes bgl2, cbh2 and eg1 were cloned by PCR. By conducting sequence alignment analysis with the reported data it was found that the homology of same cellulase genes between strain FU05 and other Trichoderma were: 91% to bgl2 (AB003110) from Trichoderma reesei; 99% to cbh2 (DQ504304) from Trichoderma koninqii and 95% to eg1 (X60652) from Trichoderma longibrachiatum. Furthermore, the corresponding amino acid sequences were also quite similar. By means of PROSITE motif search, the locations of N-glycosylation site, cellulose-binding domain and conserved domains of glycosyl hydrolases family in the corresponding protein were confirmed.