Establishment of Gel Electrophoresis for the Analysis of Peroxidases in Photosynthetic Bacteria Based on Genetic Algorithm

Abstract:

To establish the acid polyacrylamide gel electrophoresis (A-PAGE) analyzing method of peroxidase isoenzymes in photosynthetic bacteria. According to the number and the grey scale of bands, the conditions of the A-PAGE were optimized by the orthogonal design and the genetic algorithm. The results indicated that when the Triton X-100 concentration in the gels was 0.31%, the ultrasonic time was 19 min, the Triton X-100 concentration in the samples was 0.79%, the clear peroxidase isoenzyme zymograms of Rhodopseudomonas palustris were obtained, when the Triton X-100 concentration in the gels was 0.27%, the ultrasonic time was 16 min, the Triton X-100 concentration in the samples was 0.76%, the clear peroxidase isoenzyme zymograms of Rhodobacter sphaeroides were obtained. The paper set up a simple and rapid method for the analysis of peroxidase isoenzymes in photosynthetic bacteria.