Study of a Novel Strain PW0852 Producing α-Glucosidase Inhibitor

Abstract:

Actinomycete strain PW0852 with high production of α-glucosidase inhibitor was isolated from soil samples. Based on the results of morphological, physiological, chemotaxonomic characteristics and 16S rRNA analysis, strain PW0852 was placed within the genus Streptomyces and identified as the species Streptomyces lavendulae. After fermentation in a 10 L fermentor, α-glucosidase inhibitors were accumulated in the harvested broth of strain PW0852. The mixed-type α-glucosidase inhibitor GIB852 we obtained was isolated and purified after ultra-filtration, nano-filtration, resin-adsorption and desiccation. GIB852 could efficaciously inhibit human pancreatic α-amylase and α-maltase glucoamylase. The inhibition of maltase is stronger than the same dose of the commercial α-glucosidase inhibitor miglitol 28.7 times, while the inhibition of α-amylase is weak. GIB852 could significantly depress blood glucose levels in mammalian systems and be developed towards a possible therapeutic agent for diabetes.

Key words:Streptomyces lavendulae, Polyphasic taxonomy, Fermentation, α-glucosidase inhibitor