Verification and Practical Application of BDV Nucleoprotein FQ RT-PCR Kit

Abstract:

In order to verify the Fluorescent-Quantitation PCR kit specific for Borna disease virus (BDV) nucleoprotein, compare molecular beacon with common probe and study its practical application, the sensitivity, distinctness, repeatability and stability were tested on BDV and non-BDV infected cells, as well as non-infected controls. Human and animal samples were also tested by the kit. The lowest quantity of virus RNA detected by the kit was 2.5×101, which corresponded to 1 virus copy. No false positive result was found. The coefficient of variation (CV) of different batches was less than 0.7, and the CV between normal kits and the kits treated by accelerating decomposition was less than 2. The morbidities of human and animal detected by the kits were 3.6% (human), 4.2% (swine) and 1.5% (horse). The sensitivity and distinctness of this kit are better than common probe kit. All of the results indicate that this kit is an efficient tool in fundamental research, clinical detection and epidemiological investigation of BDV.

Key words:Borna Disease Virus, Real time PCR, Nucleoprotein