Antioxidant Activity of Exopolysaccharide from Rhizobium sp. N613

Abstract:

We investigated the reducing capacity of Caragana rhizobium exopolysaccharides (REPS) by the system of potassium ferricyanide and ferric chloride, determined the hydroxyl radical scavenging capacity of REPS by H2O2/Fe2+ system, measured the Superoxide radical scavenging capacity of REPS by the pyrogallol system, and we also determined its protective effects on H2O2 in hemplysis of erythrocytes and lipid per-oxidation of liver homogenate in rat. The results indicated that REPS has reductive capacity to oxides, the hydroxyl radical scavenging capacity of REPS was 35.46% (at the concentration of 2 mg/mL), the Superoxide radical scavenging capacity of REPS was 36.84% (at the concentration of 0.78 mg/mL), the protective effects of REPS on H2O2 induced hemolysis of rat erythrocytes was 43.84% (at the concentration of 1.14 mg/mL), and the protective effects on lipid per-oxidation of liver homogenate in rat was 34.46% (at the concentration of 1.14 mg/mL).

Key words:Exopolysaccharide from Rhizobium sp. N613, Reactive oxygen species, Lipid per-oxidation, Antioxidant activity