Diversity and Antimicrobial Activities of Endophytic Actinomycetes Isolated from Camptotheca acuminata Decne.

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Diversity and Antimicrobial Activities of Endophytic Actinomycetes Isolated from Camptotheca acuminata Decne.
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                        ZHU Wen-YongZHU Wen-Yong
1. Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, China
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LI JieLI Jie
1. Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, China
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ZHAO Guo-ZhenZHAO Guo-Zhen
1. Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, China
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HUANG Hai-YuHUANG Hai-Yu
1. Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, China
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QIN ShengQIN Sheng
1. Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, China; 2. The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou Normal Universit
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ZHAO Li-XingZHAO Li-Xing
1. Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, China
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XU Li-HuaXU Li-Hua
1. Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, China
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Abstract:

Ninety endophytic actinomycetes were isolated from surface-sterilized samples of Camptotheca acuminata Decne. collected from Yunnan University. Results of 16S rRNA gene sequences analysis showed that they belong to 10 genera, 6 families. Among them, 33.4% of endophytic actinomycete cultures presented antimicrobial activity. The polyketide synthasesⅠ(PKS-I), polyketide synthases Ⅱ(PKS-II) and non-ribosomal peptide synthase (NRPS) sequences were respectively detected by PCR in 28.9%, 44.4% and 48.9% of tested strains.

Key words:Camptotheca acuminata Decne., Endophytic actinomycetes, Diversity, Activeties

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ZHU Wen-Yong, LI Jie, ZHAO Guo-Zhen, HUANG Hai-Yu, QIN Sheng, ZHAO Li-Xing, XU Li-Hua. Diversity and Antimicrobial Activities of Endophytic Actinomycetes Isolated from Camptotheca acuminata Decne.[J]. Microbiology China, 2010, 37(2): 0211-0216

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