Isolation, Identification of an Endophytic Taxol-producing Fungus Obtained from Taxus Chinensis

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Isolation, Identification of an Endophytic Taxol-producing Fungus Obtained from Taxus Chinensis
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                        GENG ZhiGENG Zhi
School of Biology Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China
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LIU Kai-HuiLIU Kai-Hui
School of Biology Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China
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ZHAO Yun-XinZHAO Yun-Xin
School of Biology Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China
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LI Juan-HuaLI Juan-Hua
School of Biology Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China
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DENG Bai-WanDENG Bai-Wan
School of Biology Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China
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PENG HaoPENG Hao
School of Biology Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China
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Abstract:

This study investigated the endophytic fungi associated with Taxus chinensis growing in the Qinba Mountains. The strain LB-10 was found to produce taxol in vitro with a yield of 846.1 μg/L, detected by UV, HPLC, and HPLC-MS. The strain LB-10 was grouped into Metarhizium anisopliae based on the morphological traits and ITS sequencing.

Key words:Endophytic fungi, Taxol, Metarhizium anisopliae

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GENG Zhi, LIU Kai-Hui, ZHAO Yun-Xin, LI Juan-Hua, DENG Bai-Wan, PENG Hao. Isolation, Identification of an Endophytic Taxol-producing Fungus Obtained from Taxus Chinensis[J]. Microbiology China, 2010, 37(2): 0199-203

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