The Method Study of Shewanella decolorationis S12 Reduce Different Electron Acceptors with Anaerobic Fermentor Culture

Abstract:

Shewanella decolorationis S12 is a strain which can perform anaerobic respiration by using various electron acceptors under the anaerobic environment. In this study, to obtain the enough biomass and satisfy the need of scientific research such as membrane proteomics, we used the inorganic small molecule (sodium nitrate), metal ion (ferric citrate) and organic macromolecule (azo dye amaranth) as sole terminal electron acceptors, using different concentrations of electron donor and carbon source for anaerobic stationary culture and anaerobic fermentor culture of Shewanella decolorationis S12. The cells were cultured by fed-batch cultivation mode to confirm the optimal concentrations of electron donor and carbon source, finally the method of anaerobic fermentor culture for S12 was established. Comparing to the traditional anaerobic stationary culture method, anaerobic fermentor culture method not only ensured a high rate mass-transfer efficiency resulting in the effective reduction of electron acceptors, but also greatly increased the cell growth density, the max cell growth densities were increased to 325, 304, 369 times and cell growth times were decreased 26.5%, 17.6%, 7.5% separately. This method provided an effective way to culture a large number of cells and protein under anaerobic respiration condition. The procedure described above would be significance for the studies which need biomass cultivation of Shewanella genus bacteria and other anaerobic microorganisms under fully controlled conditions.