The Partial Genome cDNA Sequence of a Novel dsRNA Virus from Lentinus edodes and the Virus Detected by RT-PCR

Abstract:

In this paper, a mycovirus was isolated from an edible mushroom, Lentinus edodes, in China. The virus particle is bacilliform with a size of 20 nm× (100?200) nm and contains a dsRNA genome about 8.0 kb. A fragment with 1457 bp of virus genome cDNA was cloned and sequenced (Accession No. GQ372842). This partial genome sequence has no obvious homology with known nucleic acid sequences in GenBank, which suggested that the virus may be a novel mycovirus. To detect the virus from Lentinus edodes in laboratory or field, we developed a convenient and effective RT-PCR method. With this method, the specific RT-PCR product was successfully amplified from the abnormal mycelia.