Distribution and Diversity of Acidobacteria in Tropical Rain Forest Soil of Xishuangbanna

Abstract:

Acidobacteria is widely distributed in the nature and plays important roles in various ecosystems. In this study, soil samples were collected from Xishuangbanna Tropical Forest. Community DNA were directly extracted and amplified with the specific primers targeting the 16S rRNA genes of Acidobacteria to construct 16S rRNA gene libraries. The random clones from the libraries were screened and sequenced. Phylogenetic relationship of Acidobacteria in this specific ecosystem was built up. The results showed that members of the identified Acidobacteria belonged to five major groups: Gp1, Gp2, Gp3, Gp5 and an unclassified Acidobacterium. Among them, Gp1 was the most predominant group, about 50%-80%, Gp2 was followed by 12%-25%. Among the samples, the community structure of Acidobacteria was consistent. This study discovered the high diversity of Acidobacteria populations distributed in the soil ecosystems to adapt their specific environments.

Key words:Tropical forest, Red soil, Acidobacteria, RFLP analysis, Ecologically diverse