Isolation and Characterization of Bacteriophages of Vibrio cholera from the Red Tide Waters in Shenzhen

Abstract:

Using Vibrio cholerae seperated from the intestines of abalones as host, ten bacteriophages of Vibrio cholera were isolated by double-layer plate method from sea water sample from the Red tide at Dameisha of Shenzhen in 2004. Three broad lysis spectrum strains were found by detecting microbiological assay, RTD, the character of plaque morphology under electron microscope and the lysis spectrum. PsaA and PsaH were selected among all the strains as the representation to study the biological characteristics. The results proved that heat inactivation, pH, ultraviolet rays and sodium citrate restrict the ability of lysis to some dgree, additionally, magnesium perhaps have advantage on lysing. Furthermore, this study provide us further theory of researching on microbial ecological agents for eliminating Vibrio cholerae and diagnosising on food borne Vibrio cholerae using bacteriophage.