Screening and Identification of a Bacillus Strain Producing Butanol

Abstract:

A facultative anaerobic butanol producing bacteria named C2 was isolated from soil planted with rehmanniae. C2 could produce 17.17 g/L total solvent (acetone, butanol and ethanol, ABE) in a 7% corn mash and the butanol yield reached 11.2 g/L amounting to 65.2% of the total solvent. Using corn straw hydrolysate as raw material (total sugar 25 g/L), 3.64 g/L total solvent was produced, among which 72.3% was butanol, reached to 2.63 g/L. According to the analysis of morphology, physiological and biochemcal characteristics and 16S rDNA sequence, this bacteria strain was identified as Bacillus sp., and was most related to B. vallismortis, B. Atrophaeus and B. mojavensis.