Properties of Whole-cell Lipase from Burkholderia sp. ZYB002 with Organic Solvent Tolerance

Abstract:

The lipase-producing strain ZYB002 with broad-spectrum organic solvent-tolerance was identified as Burkholderia cepacia complex by the recA sequence. The optimal pH and temperature for lipolytic activity of the cell-bound lipase from Burkholderia sp. ZYB002 was 8.0 and 65°C, respectively. It was stable at temperature up to 70°C and retained 79.2% of its original activity for 1 h. The lipase was highly stable in the pH range from 3.0 to 8.5 for 6 h. Ca2+, K+, Na+, NO3?, etc. ions stimulated its lipolytic activity, whereas Zn2+ ions caused inhibition. The cell-bound lipase was also relatively stable in n-butanol at a final concentration of 50% (V/V) for 24 h. However, the lipase was strongly inhibited in triton X-100 at a final concentration of 10% (V/V). The cell-bound lipase with thermal resistance, alkaline resistance and organic solvent resistance showed its’ great potential in various industrial application fields.

Key words:Burkholderia sp., Organic solvent tolerance, Whole-cell lipase, Biochemical characterization