Application of Several PCR Methods in Cloning Unknown Genes of Duck Enteritis Virus

Abstract:

Degenerate PCR, modified targeted gene walking PCR, modified thermal asymmetric interlaced PCR and Long-PCR were performed with genomic DNA of duck enteritis virus as a template and three fragments (5350 bp, 11083 bp, 2905 bp) of DEV genome were obtained. DNA sequence analysis revealed nine open reading frames encoding UL3, UL4, UL5, UL25, UL26, UL27, UL28, and UL30 gene of DEV, respectively. These sequences have been submitted to GenBank with accession numbers from EF554396 to EF554402. Joint application of several PCR techniques is efficient in cloning unknown genes of duck enteritis virus.