Optimization of Conditions in Decolorization of Direct Fast Turquoise Blue GL Catalyzed by Laccase from Ganoderma lucidum

Abstract:

The decolorization experiments of direct fast turquoise blue GL were conducted with the crude laccase from Ganoderma lucidum in submerged fermentation. The optimum decolorization conditions were determined. The results showed that the crude laccase from Ganoderma lucidum had distinct decolorization efficiency on direct fast turquoise blue GL. The optimum pH was 3.0, The optimum temperature was 40°C, The optimum enzyme dosage was 40 U/mL, the optimum dye concentrations was 60 mg/L. Under the best conditions optimized above, the decolorization rate of direct fast turquoise blue GL was 94.3% after 70 min catalyzation with the laccase from G. lucidum. Our results suggested that the laccase from Ganoderma lucidum could be very useful in the textile-dye decolorization and waste-water purification.