Application of Enterobacter sakazakii chromogenic medium

Abstract:

Enterobacter sakazakii is an opportunistic pathogen which has been isolated at low levels from powdered infant formulas. As there are deficiencies as more time in the general method of isolation and identification of Enterobacter sakazakii, a new chromogenic medium(HKMCES) was designed in this a assay. Through detecting the reference strains, artificeially contaminated samples and natural samples, the sensitivity, specificity, detection effect and the way of pre-enrichment of HKMCSE were studied and compared with OXCSE. The results showed that the isolation of E. sakazakii was more efficiency after adequate pre-enrichment was applied. HKMCSE had the same selectivity and highly specificity as OXCSE. In con-clusion, Enterobacter sakazakii chromogenic medium were invaluable which may shorten the detection pe-riods.

Key words:Chromogenic media, Enterobacter sakazakii, Application study