Expression of Rabies Virus Glycoprotein Gene in Saccharomyces cerevisiae

Abstract:

To obtain non-pathogenic rabies virus glycoprotein (RV-G), we expressed RV-G in Saccaromyces Cerevisiae (S. cerevisiae). In our study, tat-G fusion gene was cloned into the expression vector pYes2.0, which allows expression of a foreign gene in the yeast cells under the control of GAl1 promoter. Transformation was performed by using lithium-treated yeast cells and several Ura+ -tranformants were isolated. According to the relative mobility in SDS-PAGE, we know probably two forms (designated as yGI and yGⅡ) of RV-G analogues produced in S. cerevisiae, their molecular weights were estimated as 66 kD and 56 kD, respectively. On the other hand, there was a specific band about 56 kD shown in western blot result. Com-bining precursors’ achievements, we will draw a conclusion that trans-membrane domain (TD) and cyto-plasmic domain have a negative regulation on RV-G antigen immunogenicity in S. cerevisiae.