Expression of Pili-like Protein SSU2101 of Streptococcus suis Serotype 2 and Evaluation of Its Protective Abilities to a Mouse Model

Abstract:

To prokaryotic express pili-like protein(PLP) SSU2101 of the Chinese strain 05ZYH33 of Streptococcus suis serotype 2(S. suis 2), elucidate its immunogenicity and immunoprotection. The plp gene was amplified by PCR, and then cloned into prokaryotic expression plasmid pET32a. The recombinant plasmid was transformed into E. coli BL21 competent cells to express the recombinant PLP(rPLP). The rPLP protein was purified by affinity chromatography methods. Western blot indicated clearly that the rPLP shares strong specific immunogenicity. Animal infection experiments demonstrated that the rPLP protect mice from lethal challenge with S. suis 2 infection, which suggest rPLP might be a candidate component of S. suis 2 protein vaccine.