Endophytic Actinomycetes Associated with Medicinal Plants: Isolation, Antimicribial Activity and Identification

Abstract:

272 endophytic actinomycetes were isolated from different medicinal plants collected from tropical rainforest in Xishuangbanna of Yunnan province. By antimicrobial screening, the fermentation broth of 146 strains inhibited the tested pathogenetic strains, and 94 or 127 out of the 272 endophytic actinomycetes strains showed inhibition activity against pathogenetic bacteria or fungi. The strain YIM 61470 exhibited extensive antagonism against pathogenic microbes. Based on the morphological characteristics, cultural characteristics, physiological characteristics, chemotaxonomic data and 16S rRNA gene sequence similarity analysis, strain YIM 61470 was identified as Streptomyces hydrogenans, a known species of the genus Streptomyces.