A Penicillium sp. XGH2321 Isolated from the Rhizospheric Soil of Rhizophora stylosa Griff and Its Antibacterial Activity

Abstract:

The isolate XGH2321, which isolated from the rhizospheric soil of Rhizophora stylosa Griff in Dongzhaigang Mangrove Nature Reserve in China, was identified as a fungus in the genera of Penicillium based on its morphological characteristics and internal transcribed spacer(ITS) sequence analysis. After in-oculated in the modified CzapeK-DoX medium(consisted of 4% corn steep, 0.3% NaNO3, 0.05% KCl, 0.1% K2HPO4, 0.05% MgSO4, pH 7.4, 9% salinity), and cultured under the condition of 28?C in a rotary shaker at 160 r/min for 7 days, the extracts of ethyl acetate and water-soluble from the fermentation broth showed the apparent antibacterial activities against the growth of Staphylococcus aureus, Sarcina lutea, and Bacillus subtilis with the minimum inhibitory concentration (MIC) of 800 μg/mL and 400 μg/mL, respectively. While at the same time, these two extracts could also suppress the growth of the plant pathogen, Rhizoctonia solani with MIC of 400 μg/mL and 200 μg/mL, respectively.