Identification and AHLs Detection of Dominant Bacterium of Sea Cucumber (Apostichopus japonic) Infected with the Skin Ulceration Syndrom

Abstract:

The skin ulceration syndrome of sea cucumber is a kind of desease induced by bacterium. In order to investigate the bacterium of infected sea cucumber and detect the N-acyl-homoserine lactones(AHLs) se-cretion of the bacterium, 7 bacterial strains were isolated from the infected sea cucumber. These strains were identified by physiological-biochemical characteristics and 16S rDNA sequence. Results show that strain C6 belongs to Tenacibaculum, strain 4 belongs to Shewanella putrefaciens group, strain TB belongs to Vibrio, strain BP2, BP3, BP4 and BP6 belong to Pseudoalteromonas, respectively. AHLs were detected with strain Agrobacterium tumefaciens KYC55. Among these bacterial strains, strain C6, 4, TB, BP3 and BP4 can se-cret AHLs, while strain BP2 and BP6 can’t. And the AHLs activity differs, from the highest to the lowest are 4, TB, BP4, BP3 and C6.