Fermentation Conditions of Producing Staurosporine Strain H41-38

Abstract:

This paper focused on stausporine production by cultivation of strain H41-38. Results could be summarized as follows. The optimal condition for the shake flask cultivation for this strains was 30°C、96 h. Results indicated that the best carbon and nitrogen sources were cornstarch and yeast powder. With the method of fractional factorial design, it was found that yeast powder and crude salt were the important factors, and their optimal contents were determined. Under the optimal conditions, the calculated maximal stausporine was 252.31 μg/mL, while the experimental value of shake flask was 251.28 μg/mL. The titer was 4.33 times more than using the original culture medium. After cultivating in a 30 L fermentor (300 r/min、30°C, Vair=10 L/min~15 L/min) for 84 h, the titer was 286.44 μg/mL, which titer was 45.16 μg/mL higher than the shake flask cultivation, and curves of parameter provided useful information and data for the industrial production of stausporine.