Cloning and Sequence Analysis of Azoreductase Gene from the Strain AZR

Abstract:

The result of 16S rRNA analysis showed the strain AZR belonged to Staphylococcus cohnii. According to three azoreductase’s gene sequences from Staphylococcus in GeneBank, a pair of primers were designed and the azoreductase’s gene was amplified from the strain AZR’s genome successfully. The sequence contained a complete 567 bp ORF (Open Reading Frame) encoding the azoreductase, consisting of 188 amino acids. Blast in GenBank indicated that it is a novel gene, and it has been deposited with the GenBank Data Libraries under accession number EU849488. The characteristics of the protein coded by this gene were ana-lyzed by bioinformatics. The result showed that this protein belonged to the flavin protein family and was a kind of NADPH-depedent FMN reductase, and it was stable, pI 5.75, located in cytoplasm, and composed of Alpha helix, extrended strand random coil. There was a srong transmembrance domain and contained some conserved sites such as protein dinase C-phosphorylation.