Isolation, Identification of a Sulfate Reducing Bacteria D11 and Its Phylogenetic Analysis

Abstract:

A sulfate-reducing bacteria strain, designated D11, was isolated from an anaerobic baffled reactor treating waste water containing sulfate. The cells of strain D11 were found to be Gram-negative, non-spore-forming, slightly curved rods, 0.6 μm~0.8 μm wide and 1.8 μm?~3.3 μm long, motile by a single polar flagellum, catalase positive and oxidase negative. The pH range for growth was 6.0~8.0 (optimum pH 7.0) and the temperature range for was 25°C~37°C (optimum 30°C). Strain D11 can use glucose, fructose, acetate, lactate, alcohol and propanediol as sole carbon source for growth and did not use glycerol, butanol, succinate and malate. The G+C content of the DNA was 62.7 mol%. Phylogenetic analysis based on 16S rRNA and DSR gene sequence showed strain D11 was closely related to the type strain of Desulfovibrio vulgaris Hildenborough and Desulfovibrio vulgaris DP4 (99% sequence similarity).