Isolation and Identification of Bovine Parainfluenza Virus Type3

Abstract:

One virus strain was isolated from the nasal secretion of a bovine suspected to be respiratory tract disease at a beef farm in Heilongjiang province. The virus could grow well and produce typical cytopathic effect in MDBK cells. Bovine parainfluenza virus type3(BPIV3) has been identified by a series of systematic identification such as physicochemical features, RT-PCR, and sequence analysis. As a result, the virus is an RNA virus, which is of hemagglutination and is very sensitive to ether and chloroform, no resistance to heat and acid. The homology of the nucleocapsid(N) gene sequence of the isolate and of GenBank is 82.6% to 99.1%. The isolated virus was confirmed as Bovine parainfluenza virus type3 named BPIV3 DQ1 strain. Specific primers were designed and synthesized according to the reported N protein gene sequence of Bovine parainfluenza virus type3 in GenBank. The size of 704 bp fragment was amplified and 10 TCID50/100 μL of viral nucleic acid was detected.