Genotype Analysis of Cryptoccocus neoformans Clinical Strains in Mainland China

Abstract:

We analyzed the genotype of 120 Cryptococcus neoformans and 9 Cryptococcus gattii strains isolated from cryptococcosis patients residing in 16 provinces of mainland China, using methods of DNA fingerprint, IGS sequence, MLST and construction of dendrogram based on M13 fingerprint. 120 serotype A strains exhibited an identical M13-based VNI subtype, which was distinguishable from the reference VNI molecular type, MLST also showed the same result, for convenience we named this unique genotype VNIc. (GACA)4 and URA-RFLP could not separate VNIc from other subtype of VNI. The 9 serotype B strains of C. gattii portrayed a typical VGI molecular type. This research showed that VNIc is responsible for the majority of clinical cryptococcosis in China.