PCR-SSCP Analysis of the Pronucleus Microbial Communities in Luzhou-flavor Daqu

Abstract:

PCR-SSCP (single-strand conformation polymorphism) was used for studying the community changes of pronucleus microorganisms in various fermenting stages of Luzhou-flavor Daqu. The results showed: (1) The pronucleus microorganism’s community was similar and also had the polymorphism in each simple of various fermentation stage; (2) Different microflora had complex ecology effects of coordination and the restriction; (3) The diversity indexes of different stage of Daqu microorganisms were around 1.69~2.01, and the composition of them was stable relatively; (4) The similarity indexes were 0.67~1.00, and much higher in the approaching stages.