Identification of a New Planococcus Strain and Analysis of Its Indigenous Plasmids pPCZ1 and pPCZ2

Abstract:

A new strain ZOYM was isolated from deep ocean of China and its 16S rRNA gene sequence showed high homology with that of the other published Planococcus species. Plasmids DNA were isolated and multiple bands were detected on an agarose gel, and two small of them, designated pPCZ1 and pPCZ2, were further cloned and sequenced. The complete nucleotide sequences of pPCZ1 and pPCZ2 consisted of 4738 and 7975 base pairs, encoding 3 and 8 proteins, respectively. The predicted replication proteins (Reps) of pPCZ1 and pPCZ2 showed high homology with that of Staphylococcus plasmid pSCFS1 and Bacillus plasmid pBM19, respectively. In additional, the Reps of pPCZ1 and pPCZ2 displayed homology with each other. No conserved dso and sso of the rolling-circle plasmids were found on pPCZ1 and pPCZ2, while multiple direct-repeats and AT-rich sequences were found on upstream of the rep genes, suggesting that replication of the two plasmids might be in a mechanism of theta-type. This is first report on the complete nucleotide sequences of Planococcus plasmids pPCZ1 and pPCZ2.