Primary Identification, Capability of Phosphate-Solubilization and Optimization of Medium of Some Microorganism from Mangrove

Abstract:

The genetic indentification of 16S rDNA or ITS, capability of phosphate-solubilization and pH of medium, and optimization of medium of some microorganism isolated from mangrove were investigated in this study. The result showed that the fungi normally had much higher capacity to dissolve the inorganic phosphate than the bacteria, the capacity of the fungi was closely correlated to the pH of medium, but the relationship was weak for the bacteria. It was illustrated by single factor experiments that the optimal carbon and nitrogen sources were maltose and urea respectively. The orthogonal design was employed in testing the optimum composition of medium composed of 5 g/L maltose, 0.05 g/L urea, 5 g/L NaCl, pH 5. In this optimal medium, the effectively enrichment of bacteria could reach up to 6.06×109 CFU/mL under 30°C for 48 hours cultivation.

Key words:Mangrove, Phosphate-solubilizing microorganism, Phosphate solubilization capacity, Optimized culture