Research on Conditions of Tannase Production from Aspergillus Niger B0201 Under Solid-state Fermentation Using Gallnut

Abstract:

According to the comparative research of conventional sterile fermentation and uncooked material fermentation, the conditions of tannase production from Aspergillus Niger B0201 under solid-state fermentation using gallnut was studied. The results showed that the liquid?solid ratio of 1.6: 1, temperature of 30°C, initial pH of 6.0 were the optimal conditions for tannase production under uncooked material fermentation with gallnut content of 20% and (NH4)2SO4 for nitrogen sources. Under this condition, tannase activity reached to 51.2 U/gds after 96 h of cultivation, which was 3.6 times as the conventional sterile fermentation. The results above revealed that under uncooked material fermentation for tannase production was a feasible method with high efficiency.

Key words:Aspergillus niger, Tannase, Uncooked material fermentation, Gallnut, Solid-state fermentation