Molecular methods and fluoroscopic techniques suggest that rich microbial diversity exist in the marine environment, but less than 1% of these microbes can be cultured in the laboratory conditions, and that the cultivable dominant species were even less. This limitation has long been a barrier to the development of environmental microbiology and the utilization of marine resources. In the past decade, novel methods for culture and detection of these uncultured marine microbes have successfully applied to obtain several conventionally-uncultured microbes including those from extreme environments. Those progresses have inspired researchers greatly. Developments in the research of marine microbial resources are an important basis for the study of the micro-world and deserve increasing scientific attention.
TIAN Tian, LI Dong-Mei, DAI Shi-Kun, YIN Ke-Dong, SUN Hui-Min, LI Xiang. Culture Methods of the Oligotrophic Marine Microbes[J]. Microbiology China, 2009, 36(7): 1031-1039
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