Identification of Halorubrum sp. CY Strain and Primary Study on the Basic Characteristics of Extracellular Amylase

Abstract:

An extracelluar amylase production extremely halophilic archaen was isolated from a salt ore of Yipinglang of Yunnan province. Based on the morphology, characteristics of biologic and chemical and analyzing of 16S rRNA sequence, the strain was identified as genus of Halorubrum, and named Halorubrum sp. CY. The rude characteristics of the extracelluar amylase determined. It had optimal activity at pH 6.0 and 60oC. Zn2+, Cu2+, Al3+, SO32- could promoted the activity of amylase and Mn2+ might inhibit the activity of extracelluar amylase.