Inhibition of Antibacterial Active Substances from Paenibacillus polymixa Cp-S316 on Escherichia coli and Breeding of Higher-yield Mutant

Abstract:

The effect of antibacterial active substances from Paenibacillus polymyxa Cp-S316 on the integrity of Escherichia coli K12 membrane was studied by determining the variation of OD260 values of bacterial suspensions. The results showed that the antibacterial substances could damage the Escherichia coli K12 membrane which leaded to the leakage of RNA, DNA and other cytoplasmic macromolecules. As an original strain, Paenibacillus polymyxa Cp-S316 was treated by ultraviolet mutagenesis. The mutated strain of A17 was obtained by prescreening of antibacterial active substances, screening and re-screening of shake flask culture. Compared with Cp-S316, the yield of antibacterial substances obtained from mutant A17 was increased by 91%. The subculture experiments indicated that the hereditary characteristic of high productivity of Paenibacillus polymyxa A17 was stable.