Analysis on RAPD Genetic Polymorphism of HIV Related Oral Candida Species

Abstract:

60 Candida strains were analyzed with randomly amplified polymorphic DNA(RAPD) from HIV-infected patients’ oral cavity. The results showed that RAPD-PCR fingerprinting appeared from zero to five bands whose size rang from 300 bp to 2 kb with P2 primer, Candida albicans have three characteristic bands of 300 bp, 400 bp and 600 bp in size, non-Candida albicans have the similar bands. They can be classified five genetic clusters and 14 genotypes by Cluster Analysis, two Candida albicans(P385&P403) with fluorocytosine-resistance were classified into C1 genotype for “Squared Euclidean Distance” being 0.115, one Candida albicans(P321) and one Candida glabrata(P522) who were resistant against Amphotericin B were classified into D1 genotype for “Squared Euclidean Distance” being 0.221. So, an abundant genetic polymorphism appeared in Candida species related HIV, RAPD could be as a reference for genotype of Candida albicans; different Candida speices had some special bands, different Candida strains had similar bands in the same species; it is perhaps that some genotypes were relevant to drug resistance.

Key words:Genetic polymorphism, Susceptibility, Candida albicans, HIV