Optimization of Fermentation Medium for S-adenosylmethionine Production by Candida sp. YQ5

Abstract:

The optimized cultural medium of fermentation for Candida sp. mutant strain YQ5 to produce S-adenosylmethionine was studied. The results of single factor experiment showed that the most favorable pH value, carbon source, nitrogen source organic nutrient is 6.0, 8% sucrose, 1.5% tryptone and 2% yeast extract, respectively. As to inorganic salts, MgSO4·7H2O, CaCl2, FeSO4·7H2O, CoCl2, CuSO4·5H2O, H3BO3 could improve accumulation of the intercellular SAM. The ingredients of the culture medium are also optimized by the orthogonal experiment. Fermentation for 48 h under the optimal condition resulted in AdoMet production at 1740.0 mg/L.