Isolation, Identification and Degradation Characteristics of a Slight Halophilic Denitrifying Bacteria

Abstract:

A slight halophilic denitrifying bacteria (designated GYL) was screened from the activated sludge which was used to treat high-salinity wastewater. According to the results of morphological observation, physiological and biochemical test, sequence analysis of the 16S rDNA, strain GYL was identified as Halomonas sp.. This strain could survive at 10% salinity and the optimal salinity range for growth was 2%~7%. The suitable pH value for growth was 7.5~8.5 and sucrose was the most effective carbon source. The nitrogen removal efficiency exceeded 80% when the temperature ranged from 25°C to 30°C. Meanwhile heterotrophic nitrification characteristics of this strain were measured. Results showed that this strain was able to realize SND and ammonia removal rate was 98.3%. It showed that this strain could perform the whole process of bacteria denitrification independently.