Analysis and Identification of Pathogenic Bacteria by MALDI-TOF Mass Spectrometry

Abstract:

Based on Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI- TOF-MS) method for discrimination of pathogenic bacteria was developed. Established the standardization method for MALDI-TOF-MS analysis of bacteria and affecting elements of the analyzing result. The results obtained from twelve plants pathogenic bacteria of different genus, species and subspecies were analyzed by using MALDI-TOF showed that, MALDI-TOF-MS could be rapid and reliable identification of bacteria at the level of genus, species, and strains. Analytical process is simple, high sensitivity are advantages of this strategy.

Key words:MALDI-TOF-MS, Bacteria mass finger-printing, Identification, Pathogenic bacteria