Cloning and Localization of the Ser/Thr Protein Kinase Cpn0148 from Chlamydia pneumoniae

Abstract:

Chlamydia pneumonia(Cpn) is thought to be one of the important pathogens which are associated with coronary heart disease. It can induce the activation of host signaling pathways to maintain the survival and metabolism of itself in the cell, and finally leads to disease. The ORFs of Cpn0148 encoding eukaryote-like serine/threonine protein kinase was amplified from Chlamydia pneumonia serovar AR39 genomic DNA. The amplified product was cloned in pGEX-6p and expressed in XL-1blue. DNA sequencing showed that the whole ORF is about 1860 bp, encoding a protein with 619 amino acid. The molecular weight of Cpn0148 is about 70 kD. Polyclonal antibody was obtained from immune BALB/c mice with the Cpn0148-GST fusion protein, and the expressing of Cpn0148 was detected on the bacteria organism by immunoflouresence. The finding of Cpn0148 protein is very helpful foundation for further study of the host-bacteria interaction.