Research of Screening Method of the High Bio-oil Production Microorganisms

Abstract:

Fat Particles Counting(FPC), Yeast Mud Dying by Sudan III(YMDSIII), Culture of Lacking Carbon(CLC) were compared and approached in the essay. The results show that YMDSIII is simple and direct, its results are better interrelated with oil-producing quantity of yeast. This method is a relatively ideal screening method on choosing oil-producing microorganisms. CLC is relatively accurate, but it is too tedious. By comparison, FPC is simple and convenient, but it is short of accuracy. When it is used, it must consider the size of fat particles. By screening, cellular oil content reached 54.25% (W/W).

Key words:Bio-oil, Screening methed, Fat particles, Oil contents