Pure culture of Phlebopus portentosus was inoculated in the roots of coffee tree. The results indicated that the young fruit bodies would come out around the rhizomes of host tree after inoculation in 30 to 90 days, single or cluster, 3 to 4 days for mature, weight 20.0 g to 62.0 g. Brown rhizomorph and hyphae can be seen on the seedlings`rhizome, main root and side root while nothing is on the tip of the root.It was found that rhizomorph on the surface of roots would die after inoculation in 90 days in pot.
JI Kai-Ping, HE Ming-Xia, ZHANG Chun-Xia, LIU Jing, WANG Wen-Bing, HOU Jian-Yong. Semi-artificial Simulate Cultivation of Phlebopus portentosus and the Durability of Hyphae on Host Roots[J]. Microbiology China, 2009, 36(3): 0377-0382
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