Analysis of Bacterial Diversity During the Processing of Brassica juncea coss var. tsatsai by the Culture-independent and Denaturing Gradient Gel Electrophoresis (DGGE) Methods

Abstract:

Both culture-dependent and culture-independent methods, denaturing gradient gel electrophoresis (DGGE) based on the sequence of 16S rRNA V3 region gene, were used to examine the total bacteria counts, bacterial diversity and community structure of different periods Brassica juncea saline water samples. The results showed that 7 genera of bacteria were identified from 19 isolated bacterial populations by the traditional isolation method, the dominant bacteria in intestine belonged to Acidobacterium. By 16S rRNA V3 region gene DGGE method, 11 distinct bands were obtained from 16S rDNA amplifications. The profile of DGGE fingerprints of different periods saline water revealed that the most dominant bacteria group was Leuconostoc in the process of the fermentation. This suggested that a combination of molecular and traditional culture methods can be used to analyze and monitor the diversity of the process of fermentation microflora effectively, and that will give us more information of microorganism diversity.