Using Mini-Tn10 Transposon System to Research the Genes Involved in Biofilm Formation in Bacillus

Abstract:

Biofilm has great influence for bacteria to resist the harm from outer environment. Its forming and development are controlled by many different genes. In this research, an insertional mutagenesis library of a wide type Bacillus amyloliquefaciens NK10.BAhjaWT was built via mini-Tn10 transposon system to find out genes involved in biofilm formation. Four such genes have been screened, in which, citB, citG, gpsA are all related to the energy metabolism. The function of another gene of yvfB is unknown. In a word, mini-Tn10 transposon system was proved to be efficient and stable in Bacillus.