Optimization of Culture Condition for Novel Biopolymer Ss Production by Sphingomonas sp. Strain TP-3

Abstract:

Sphingomonas sp. TP-3 synthesizes a type of novel biopolymer Ss with properties of thickening, pseudoplastic, gelling and emulsification. Both single factor experiments and uniform design were employed in this study to optimize fermentation conditions of strain TP-3. The optimal fermentation paramenters in shaking flask were as follow: glucose 41.2 g/L, soybean meal 2.0 g/L, NaCl 0.85 g/L, K2HPO4 1.46 g/L, MgSO4 0.12 g/L, MnCl2 0.0075 g/L, FeSO4 0.002 g/L, initial pH 7.0, culture temperature 27°C, rotating speed 180 r/min. The yield of biopolymer Ss reached 21.5 g/L. As a low-cost biopolymer, Ss has wide prospect for application in oil recovery.

Key words:Sphingomonas sp., Biopolymer, Fermentation conditions, Uniform design