Isolation and Identification of One Marine Actinomycete Strain Exhibiting Antitumor Activity

Abstract:

The fermentation broth of one actinomycete strain ACMA006 strongly inhibited growth of many tumor cells and some microorganisms, but its cytotoxicity to human normal cells were weak. Strain ACMA006 grow well on most tested media, producing exuberant vegetative hyphae and aerial hyphae. Its optimization temperature is 28°C. Phylogenetic analysis based on 16S rDNA sequence showed that strain ACMA006 was closely related to one of the genus Streptomycetes (S.cavourensis subsp. washingtonensis) with 16S rDNA sequence similarity values of 100%, but had many differences in other features including its morphology, physiological and biochemical characteristics. The preliminary study supported the view that the strain ACMA006 represented a new strain of the S.cavourensis subsp. washingtonensis.

Key words:Antitumor activity, Marine actinomycete, 16S rDNA, Phylogenetic analysis