The Variation of Genetic and Virulent of Xanthomonas oryzae pv. oryzae Strains and Mono-cell-clones from China, Japan and the Philippines

Abstract:

The primers J3, IS1112, IS1113, and ERIC were used to analyze 17 tested groups (56 strains and their mono-cell-clones) of Xanthomonas oryzae pv. oryzae from China, Japan and the Philippines. The result showed: 1) The percentage were 52.9%(J3), 23.5%(IS1112), 29.4%(IS1113) and 35.3%(ERIC) which strains and their mono-cell-clones were in one patten of each primer, respectively. 2) Percentage were 29.4% which the similarity were over 90% between strains and their mono-cell-clones of 17 tested groups, while 52.9% of dissimilarity were 30%~41%; at level of 80% similarity, 9 tested groups which strains and their mono-cell clones were in one cluster(52.9% of 17 tested groups), these results suggested there were phylogenetic relationship between the strains. Also the differentiation were exits. Such as, Philippine strains, Pxo79 and Pxo112 were distributed into 3 clusters; the dissimilarity were 41% between strains and their mono-cell- clones of Pxo79, Pxo86, Pxo99 and Pxo112. These results indicated the strains were combined by different cells. 3) There was only 11.8% of tested strains which virulence of strains were equal to their mono-cell- clones in 17 tested groups. The result indicated that there were virulent difference between strains and their mono-cell-clones. No relationship were observed between UPGMA clusters and pathotypes.

Key words:Xanthomonas oryzae pv. oryzae, Mono-cell-clones, Genetic variation, Virulent differentiation