Isolation and Identification of Thermoacidiphilic Bacteria from Apple Juice Concentrate

Abstract:

The thermotolerant bacteria which was isolated from the Apple Juice Concentrate (AJC) was investigated by comparison with the standard strain of Aliyclobacillus acidoterrestris DSM3922 using Kirin-medium acidified with malic acid. The results shows the two polluted bacteria isolated from AJC can grow under the temperature of 21°C~55°C and pH of 2.4~6.2, which was corresponded with the characteristics of the thermo-acidiphilic Alicyclobacillus spp.. Further more, the cell morphology, colony morphology, cultural characteristics and physiological characteristics tests indicated the two isolated strains of thermotolerant bacteria have obviously similar characteristics with the standard strain of Aliyclobacillus acidoterrestris DSM3922.