Screening of Antagonistic Strains to Tobacco Anthracnose

Abstract:

Special PCR primers were designed from the sequence of 16S-23S ribosomal RNA of Pseudomonas fluorescens, then these primers were used to screening 400 bacteria strains which selected from tobacco planting soil. In the experiment, 21 Pseudomonas fluorescens strains which contain Pyrrolnitrin had been identified, and 1 Pseudomonas fluorescens strain which contain 2,4-diacetylphloroglucinal and Phenazine-1-carboxylic acid had been identified, too. The results showed that the strain which contain 2,4-diacetylphloroglucinal and Phenazine-1-carboxylic acid inhibit to tobacco anthracnose.