Determination of 7β-hydroxyglycyrrhetinic Acid in Microbial Transformation of Glycyrrhetinic Acid by Mucor spinosus by HPLC

Abstract:

The major product of microbial transformation of glycyrrhetinic acid by Mucor spinosus AS3.3450 has been identified as 7β-hydroxyglycyrrhetinic acid on the basis of its spectral data. 7β-hydroxyglycyrrhetinic acid was directly determined by HPLC. Methanol and ultra pure water containing 0.03% trifluoroacetic acid was used as the mobile phase on C18 column, the UV detection wavelength was 254 nm. The maximum transformation rate observed was 712 mg 7β-hydroxyglycyrrhetinic acid/g glycyrrhetinic acid on day 9 under 27°C, 160 r/min and substrate at a concentration of 130 mg/L. Results show that the content of 7β-hydroxyglycyrrhetinic acid indicates a good linearity under these conditions with a average recovery of 98.1% and a RSD of 2.37%.